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pIW601-KmCRISPR
(Plasmid #98907)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98907 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJSK316-GPD
  • Backbone size w/o insert (bp) 5558
  • Total vector size (bp) 11093
  • Vector type
    Yeast Expression, CRISPR, Synthetic Biology
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Codon optimized Cas9
  • Species
    Synthetic; K. marxianus
  • Insert Size (bp)
    4140
  • Tag / Fusion Protein
    • SV40 (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    sgRNA expression cassette
  • Species
    Synthetic
  • Insert Size (bp)
    693

Resource Information

Depositor Comments

Easy cloning vector: Cut backbone with PspXI for Gibson Assembly with annealed primers of 20bp (up- and downstream) to insert 20bp target sequence .

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIW601-KmCRISPR was a gift from Ian Wheeldon (Addgene plasmid # 98907 ; http://n2t.net/addgene:98907 ; RRID:Addgene_98907)
  • For your References section:

    CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus. Lobs AK, Engel R, Schwartz C, Flores A, Wheeldon I. Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017. 10.1186/s13068-017-0854-5 PubMed 28652865