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pZS157 CRISPEY RT/Cas9
(Plasmid #114454)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 114454 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9, Ec86-RT
  • Species
    S. pyogenes, E. coli
  • Mutation
    S. cerevisiae codon optimized
  • Promoter GAL1-GAL10
  • Tag / Fusion Protein
    • SV40-NLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAATTAACCCTCACTAAAGG
  • 3′ sequencing primer taatacgactcactatagg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZS157 CRISPEY RT/Cas9 was a gift from Hunter Fraser (Addgene plasmid # 114454 ; http://n2t.net/addgene:114454 ; RRID:Addgene_114454)
  • For your References section:

    Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing. Sharon E, Chen SA, Khosla NM, Smith JD, Pritchard JK, Fraser HB. Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8. doi: 10.1016/j.cell.2018.08.057. 10.1016/j.cell.2018.08.057 PubMed 30245013