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pTRE-Tight-MitoTimer
(Plasmid #50547)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50547 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRE Tight
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 2516
  • Total vector size (bp) 3325
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MitoTimer
  • Insert Size (bp)
    809
  • Mutation
    F12S mutation in Timer

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer AGG CGT ATC ACG AGG CCC TTT CGT
  • 3′ sequencing primer TAT TAC CGC CTT TGA GTG AGC TGA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The F12S mutation in Timer does not affect the function of the plasmid, as described in the associated publication.

The Timer sequence is preceded by the mitochondrial targeting sequence from cytochrome c oxidase subunit 8A: MSVLTPLLLRGLTGSARRLPVPRAKIHSL

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE-Tight-MitoTimer was a gift from Roberta Gottlieb (Addgene plasmid # 50547 ; http://n2t.net/addgene:50547 ; RRID:Addgene_50547)
  • For your References section:

    MitoTimer: A novel tool for monitoring mitochondrial turnover. Hernandez G, Thornton C, Stotland A, Lui D, Sin J, Ramil J, Magee N, Andres A, Quarato G, Carreira RS, Sayen MR, Wolkowicz R, Gottlieb RA. Autophagy. 2013 Nov 1;9(11):1852-61. doi: 10.4161/auto.26501. Epub 2013 Oct 11. 10.4161/auto.26501 PubMed 24128932