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Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing.
Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK
Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
53369pSQT1601human codon optimized Csy4 and FokI-dCas9 expression plasmid
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53370pSQT1313multiplex gRNA expression plasmid
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53371pSQT834Csy4 and Cas9 nuclease expression plasmid
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53372pNW3Csy4 and Cas9 D10A nickase expression plasmid
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53373pSQT817wildtype Cas9 expression plasmid
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60071RFN_EGFP_47Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
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60072RFN_EGFP_80Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
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60073RFN_EGFP_81Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
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60074RFN_EGFP_82Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
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60075RFN_EGFP_83Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
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60076RFN_EGFP_84Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
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