Purposewildtype Cas9 expression plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53373||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
- Promoter CAG
- Cloning method Unknown
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer Bglob-pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSQT817 was a gift from Keith Joung (Addgene plasmid # 53373 ; http://n2t.net/addgene:53373 ; RRID:Addgene_53373)
For your References section:Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325