Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pHAGE EF1α dCas9-KRAB Citations (25)

Originally described in: Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.
Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
PubMed Journal

Articles Citing pHAGE EF1α dCas9-KRAB

Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Pham H, Kearns NA, Maehr R. Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3. PubMed

Associated Plasmids

CRISPR/dCas9-mediated Transcriptional Inhibition Ameliorates the Epigenetic Dysregulation at D4Z4 and Represses DUX4-fl in FSH Muscular Dystrophy. Himeda CL, Jones TI, Jones PL. Mol Ther. 2015 Nov 3. doi: 10.1038/mt.2015.200. PubMed
Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation. Shao S, Chang L, Sun Y, Hou Y, Fan X, Sun Y. ACS Synth Biol. 2017 Sep 7. doi: 10.1021/acssynbio.7b00268. PubMed

Associated Plasmids

Dynamic evolution of regulatory element ensembles in primate CD4(+) T cells. Danko CG, Choate LA, Marks BA, Rice EJ, Wang Z, Chu T, Martins AL, Dukler N, Coonrod SA, Tait Wojno ED, Lis JT, Kraus WL, Siepel A. Nat Ecol Evol. 2018 Mar;2(3):537-548. doi: 10.1038/s41559-017-0447-5. Epub 2018 Jan 29. PubMed
Identification of Epigenetic Regulators of DUX4-fl for Targeted Therapy of Facioscapulohumeral Muscular Dystrophy. Himeda CL, Jones TI, Virbasius CM, Zhu LJ, Green MR, Jones PL. Mol Ther. 2018 Jul 5;26(7):1797-1807. doi: 10.1016/j.ymthe.2018.04.019. Epub 2018 Apr 26. PubMed
Long non-coding RNA PSMB8-AS1 regulates influenza virus replication. More S, Zhu Z, Lin K, Huang C, Pushparaj S, Liang Y, Sathiaseelan R, Yang X, Liu L. RNA Biol. 2019 Mar;16(3):340-353. doi: 10.1080/15476286.2019.1572448. Epub 2019 Jan 28. PubMed
Participation of integrin beta3 in osteoblast differentiation induced by titanium with nano or microtopography. Lopes HB, Freitas GP, Elias CN, Tye C, Stein JL, Stein GS, Lian JB, Rosa AL, Beloti MM. J Biomed Mater Res A. 2019 Jun;107(6):1303-1313. doi: 10.1002/jbm.a.36643. Epub 2019 Feb 23. PubMed
Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Genga RMJ, Kernfeld EM, Parsi KM, Parsons TJ, Ziller MJ, Maehr R. Cell Rep. 2019 Apr 16;27(3):708-718.e10. doi: 10.1016/j.celrep.2019.03.076. PubMed

Associated Plasmids

Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa. Lau CH, Ho JW, Lo PK, Tin C. Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23. PubMed

Associated Plasmids

A principled strategy for mapping enhancers to genes. Kim D, An H, Shearer RS, Sharif M, Fan C, Choi JO, Ryu S, Park Y. Sci Rep. 2019 Jul 30;9(1):11043. doi: 10.1038/s41598-019-47521-w. PubMed
Epigenome editing strategies for the functional annotation of CTCF insulators. Tarjan DR, Flavahan WA, Bernstein BE. Nat Commun. 2019 Sep 18;10(1):4258. doi: 10.1038/s41467-019-12166-w. PubMed
Active and Repressed Chromatin Domains Exhibit Distinct Nucleosome Segregation during DNA Replication. Escobar TM, Oksuz O, Saldana-Meyer R, Descostes N, Bonasio R, Reinberg D. Cell. 2019 Oct 31;179(4):953-963.e11. doi: 10.1016/j.cell.2019.10.009. PubMed
Effective CRISPR interference of an endogenous gene via a single transgene in mice. MacLeod RS, Cawley KM, Gubrij I, Nookaew I, Onal M, O'Brien CA. Sci Rep. 2019 Nov 21;9(1):17312. doi: 10.1038/s41598-019-53611-6. PubMed
High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Legut M, Daniloski Z, Xue X, McKenzie D, Guo X, Wessels HH, Sanjana NE. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. PubMed

Associated Plasmids

CRISPR interference-mediated noggin knockdown promotes BMP2-induced osteogenesis and calvarial bone healing. Hsu MN, Yu FJ, Chang YH, Huang KL, Pham NN, Truong VA, Lin MW, Kieu Nguyen NT, Hwang SM, Hu YC. Biomaterials. 2020 Sep;252:120094. doi: 10.1016/j.biomaterials.2020.120094. Epub 2020 May 10. PubMed
Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells. Chang L, Li M, Shao S, Li C, Ai S, Xue B, Hou Y, Zhang Y, Li R, Fan X, He A, Li C, Sun Y. Protein Cell. 2022 Apr;13(4):258-280. doi: 10.1007/s13238-020-00794-8. Epub 2020 Nov 5. PubMed
Suppression of the NTS-CPS1 regulatory axis by AFF1 in lung adenocarcinoma cells. Yue J, Dai Q, Hao S, Zhu S, Liu X, Tang Z, Li M, Fang H, Lin C, Luo Z. J Biol Chem. 2021 Jan-Jun;296:100319. doi: 10.1016/j.jbc.2021.100319. Epub 2021 Jan 22. PubMed
LETR1 is a lymphatic endothelial-specific lncRNA governing cell proliferation and migration through KLF4 and SEMA3C. Ducoli L, Agrawal S, Sibler E, Kouno T, Tacconi C, Hon CC, Berger SD, Mullhaupt D, He Y, Kim J, D'Addio M, Dieterich LC, Carninci P, de Hoon MJL, Shin JW, Detmar M. Nat Commun. 2021 Feb 10;12(1):925. doi: 10.1038/s41467-021-21217-0. PubMed
Identifying oligodendrocyte enhancers governing Plp1 expression. Kim D, An H, Fan C, Park Y. Hum Mol Genet. 2021 Jul 6. pii: 6316230. doi: 10.1093/hmg/ddab184. PubMed
Multiple stages of evolutionary change in anthrax toxin receptor expression in humans. Choate LA, Barshad G, McMahon PW, Said I, Rice EJ, Munn PR, Lewis JJ, Danko CG. Nat Commun. 2021 Nov 15;12(1):6590. doi: 10.1038/s41467-021-26854-z. PubMed
Quantitative Comparison of HSF1 Activators. Steurer C, Kerschbaum S, Wegrostek C, Gabriel S, Hallaj A, Ortner V, Czerny T, Riegel E. Mol Biotechnol. 2022 Aug;64(8):873-887. doi: 10.1007/s12033-022-00467-3. Epub 2022 Feb 26. PubMed
A protocol for CRISPR-mediated activation and repression of human endogenous retroviruses in human pluripotent stem cells. Nair VP, Mayer J, Vincendeau M. STAR Protoc. 2022 Apr 12;3(2):101281. doi: 10.1016/j.xpro.2022.101281. eCollection 2022 Jun 17. PubMed
A Nodal enhanced micropeptide NEMEP regulates glucose uptake during mesendoderm differentiation of embryonic stem cells. Fu H, Wang T, Kong X, Yan K, Yang Y, Cao J, Yuan Y, Wang N, Kee K, Lu ZJ, Xi Q. Nat Commun. 2022 Jul 9;13(1):3984. doi: 10.1038/s41467-022-31762-x. PubMed
Identifying an oligodendrocyte enhancer that regulates Olig2 expression. Fan C, Kim D, An H, Park Y. Hum Mol Genet. 2023 Feb 19;32(5):835-846. doi: 10.1093/hmg/ddac249. PubMed
Long non-coding RNA PCAT19 safeguards DNA in quiescent endothelial cells by preventing uncontrolled phosphorylation of RPA2. Oo JA, Palfi K, Warwick T, Wittig I, Prieto-Garcia C, Matkovic V, Tomaskovic I, Boos F, Izquierdo Ponce J, Teichmann T, Petriukov K, Haydar S, Maegdefessel L, Wu Z, Pham MD, Krishnan J, Baker AH, Gunther S, Ulrich HD, Dikic I, Leisegang MS, Brandes RP. Cell Rep. 2022 Nov 15;41(7):111670. doi: 10.1016/j.celrep.2022.111670. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.