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pENTR2B-dCas9-KRAB
(Plasmid #115547)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115547 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pENTR2B
  • Backbone size w/o insert (bp) 2280
  • Total vector size (bp) 6826
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-KRAB
  • Species
    Synthetic
  • Insert Size (bp)
    4546
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR2B-dCas9-KRAB was a gift from Rene Maehr (Addgene plasmid # 115547 ; http://n2t.net/addgene:115547 ; RRID:Addgene_115547)
  • For your References section:

    Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Genga RMJ, Kernfeld EM, Parsi KM, Parsons TJ, Ziller MJ, Maehr R. Cell Rep. 2019 Apr 16;27(3):708-718.e10. doi: 10.1016/j.celrep.2019.03.076. 10.1016/j.celrep.2019.03.076 PubMed 30995470