pCSCMV:tdTomato
(Plasmid #30530)

• Purpose: mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.
• Depositing Lab: Gerhart Ryffel
• Publication: Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.1186/1471-213X-9-37.
• Citations: 42 Articles

Backbone

• Vector backbone: pCSGFP2
• Backbone size w/o insert (bp): 4100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato
• Species: synthetic
• Insert Size (bp): 1431
• Mutation: R415H
• GenBank ID: AY678269

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor, including a H415R mutation in the downstream dimer component of tdTomato. This mutation may impact tdTomato expression.