|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||91797||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Root Lab (addgene #41390)
- Backbone size (bp) 9621
Modifications to backboneFKBP F36V tag (dTAG) added to the 5' end of the gateway cassette.
Vector typeMammalian Expression, Lentiviral
- Promoter hPGK
/ Fusion Protein
- FKBP F36V (N terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsccdB resistant bacteria. 30C for liquid culture and for plates.
Copy numberHigh Copy
- Cloning method Gateway Cloning
- 5′ sequencing primer TGTTCCGCATTCTGCAAGCCTC
- 3′ sequencing primer ACAAAGGCATTAAAGCAGCGTATCC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLEX_305-N-dTAG was a gift from James Bradner (Addgene plasmid # 91797 ; http://n2t.net/addgene:91797 ; RRID:Addgene_91797)
For your References section:The dTAG system for immediate and target-specific protein degradation. Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, Yang A, Leggett AL, Erb MA, Lawlor MA, Souza A, Scott TG, Vittori S, Perry JA, Qi J, Winter GE, Wong KK, Gray NS & Bradner JE.. Nature Chemical Biology (2018) 10.1038/s41589-018-0021-8