174,398 results
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Plasmid#233210PurposeCloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with mScarlet markerDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only
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pHH21-BNA-Luc
Plasmid#187924PurposeExpresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cellsDepositorInsertReporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
ExpressionMammalianPromoterRNA polymease I PromoterAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-FL
Plasmid#190821PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags6x HisExpressionBacterialMutationCodon optimized for expression in E.coliPromoterT7Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p16 nlsGPS2
Plasmid#228093PurposeExpression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)DepositorInsertnlsGPS2
Tags6xHis-T7 XpressTag and cMycExpressionPlantPromoterp16Available SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSXneo 270(T2AG3)
Plasmid#12403DepositorInsert270(T2AG3)
ExpressionMammalianAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P07
Plasmid#124788PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenPromoterNoneAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta
Plasmid#89347Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequencesDepositorInsertJurkat TCRalpha/beta alleles and CD3e and CD3z
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMay 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AttB _EGFP
Plasmid#171597PurposeStrong EGFP fluorescence from Bxb1 landing padDepositorInsertEGFP
UseSynthetic Biology; Promoterless bxb1 dna recombin…ExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756K
Plasmid#60731PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO mScarlet
Plasmid#131002PurposeDouble floxed mScarlet under the control of Ef1a promoterDepositorHas ServiceAAV1 and AAV5InsertmScarlet
UseAAVPromoterEf1aAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T7gene1
Plasmid#165505PurposeHighly processive DNA-dependent RNA polymerase derived from T7 bacteriophageDepositorInsertT7 RNA Polymerase
UseSynthetic BiologyAvailable SinceJuly 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
actc1b-Lifeact-mKate2
Plasmid#109493PurposeMuscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertactc1b-Lifeact-mKate2
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE-GFP-Blast
Plasmid#135673PurposeDox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selectionDepositorInsertGFP
UseLentiviralAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT240
Plasmid#80414PurposeExpresses 240 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
ExpressionMammalianAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-CjCas9
Plasmid#89754PurposeExpression of CjCas9 with His tag in E.coliDepositorInsertCjCas9
UseCRISPRTags6XHis, HA, and SV40 NLSExpressionBacterialPromoterT7Available SinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-array 8
Plasmid#175580PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT480
Plasmid#80413PurposeExpresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
ExpressionMammalianAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 ermB cassette
Plasmid#117121PurposeErythromycin resistance cassetteDepositorInsertErythromycin resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…Available SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only