|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11012||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBABE puro
Backbone manufactureravailable at Addgene
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEGFR L858R
Alt nameepidermal growth factor receptor
SpeciesH. sapiens (human)
Insert Size (bp)3639
Entrez GeneEGFR (a.k.a. ERBB, ERBB1, HER1, NISBD2, PIG61, mENA)
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site SnaBI (destroyed during cloning)
- 5′ sequencing primer pBABE5' (Common Sequencing Primers)
Author has deposited the sequence of the insert to Addgene. Click on "sequence" to view. Point mutation made by using the following primers to PCR from plasmid 11011: 5'-AAGATCACAGATTTTGGGAGGGCCAAACTGCTGGGTG-3' and 5'-CACCCAGCAGTTTGGCCCTCCCAAAATCTGTGATCTT-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EGFR L858R was a gift from Matthew Meyerson (Addgene plasmid # 11012 ; http://n2t.net/addgene:11012 ; RRID:Addgene_11012)
For your References section:Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M. PLoS Med. 2005 Oct 4. 2(11):e313. 10.1371/journal.pmed.0020313 PubMed 16187797