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nls-NgAgo-GK
(Plasmid #78253)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78253 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6679
  • Modifications to backbone
    pEGFP-N1 without the eGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NgAgo
  • Alt name
    Argonaute
  • Species
    Natronobacterium gregoryi
  • Insert Size (bp)
    2712
  • GenBank ID
    AFZ73749.1
  • Promoter CMV
  • Tag / Fusion Protein
    • a nuclear localization signal (NLS) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nls-NgAgo-GK was a gift from Chunyu Han (Addgene plasmid # 78253)
  • For your References section:

    DNA-guided genome editing using the Natronobacterium gregoryi Argonaute. Gao F, Shen XZ, Jiang F, Wu Y, Han C. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3547. 10.1038/nbt.3547 PubMed 27136078