PurposeMammalian expression of Androgen receptor fused to EGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28235||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4731
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsDH5 alpha, XL10 gold
Copy numberHigh Copy
Gene/Insert nameAndrogen receptor
SpeciesH. sapiens (human)
Insert Size (bp)2760
Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn I (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Addgene's sequencing result indicates a loss of Q58 in the unstable poly-glutamine region. Our sequencing also shows that the BamHI site at the 3' end of the insert was destroyed. Please see our quality control sequence for the most accurate look at this region.
Tilley, W.D., Marcelli, M., Wilson, J.D. and McPhaul, J.M. (1989) Characterization and cloning of a cDNA encoding the human androgen receptor. Proc. Natl Acad. Sci. USA, 86, 327–331
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-C1-AR was a gift from Michael Mancini (Addgene plasmid # 28235 ; http://n2t.net/addgene:28235 ; RRID:Addgene_28235)
For your References section:Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino GN, Marcelli M, Weigel NL, Mancini MA. Hum Mol Genet. 1999 May . 8(5):731-41. 10.1093/hmg/8.5.731 PubMed 10196362