Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEGFP-C1-AR
(Plasmid #28235)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28235 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha, XL10 gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Androgen receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2760
  • GenBank ID
    NM_000044
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene's sequencing result indicates a loss of Q58 in the unstable poly-glutamine region. Our sequencing also shows that the BamHI site at the 3' end of the insert was destroyed. Please see our quality control sequence for the most accurate look at this region.

Tilley, W.D., Marcelli, M., Wilson, J.D. and McPhaul, J.M. (1989) Characterization and cloning of a cDNA encoding the human androgen receptor. Proc. Natl Acad. Sci. USA, 86, 327–331

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-AR was a gift from Michael Mancini (Addgene plasmid # 28235 ; http://n2t.net/addgene:28235 ; RRID:Addgene_28235)
  • For your References section:

    Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino GN, Marcelli M, Weigel NL, Mancini MA. Hum Mol Genet. 1999 May . 8(5):731-41. 10.1093/hmg/8.5.731 PubMed 10196362