pEGFP-C1-AR Q48
(Plasmid #86428)

• Purpose: Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region
• Depositing Lab: Michael Mancini
• Publication: Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech (Takara)
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 8026
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: New plasmid preps must be sequenced to verify (there can be loss or addition of poly-glutamine repeats).
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Androgen receptor (AR)
• Species: H. sapiens (human)
• Insert Size (bp): 2850
• Mutation: Poly-glutamine expansion, G130D
• GenBank ID: NM_000044.4
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xma I (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results found that this plasmid carries an extra Q in the poly-glutamine expansion bring the total to 49 rather than 48 and found a G130D mutation compared to NCBI reference sequences. These mutations are not known to affect plasmid function.