We narrowed to 15,165 results for: pET

1. pSLS.539

   Plasmid

   #184958

   Purpose

   Test effect of a1/a2 length on editing fabH using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
   

   Expression

   Bacterial

   Mutation

   fabH donor G954A

   Promoter

   T7/lac

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRPF4.001.169

   Plasmid

   #137233

   Purpose

   Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.

   Depositor

   Tara Davis

   Article

   Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

   Insert

   PRPF4 (PRPF4 Human)
   

   Tags

   (His)6-TEV

   Expression

   Bacterial

   Mutation

   001-169

   Available Since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLS.543

   Plasmid

   #184960

   Purpose

   Test effect of a1/a2 length on editing murF using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
   

   Expression

   Bacterial

   Mutation

   murF donor G1359A

   Promoter

   T7/lac

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLS.545

   Plasmid

   #184962

   Purpose

   Test effect of a1/a2 length on editing priB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
   

   Expression

   Bacterial

   Mutation

   priB donor G1069A

   Promoter

   T7/lac

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ppwd1.473.646.W601A

   Plasmid

   #137660

   Purpose

   Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Tara Davis, Melissa Jurica

   Article

   Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

   Insert

   ppwd1 (PPWD1 Human)
   

   Tags

   (His)6-thrombin

   Expression

   Bacterial

   Mutation

   473-646-W601A

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPD3cC

   Plasmid

   #86972

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

   Insert

   PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …

   Promoter

   T7 promoter

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1G108CP20X

   Plasmid

   #85096

   Purpose

   Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The G108C variant of PCNA1 fused to putidaredoxin
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C in PCNA1

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGS-3R112C/T180C

   Plasmid

   #85095

   Purpose

   Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Arginine 112 to Cysteine and Threonine 18…

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1G108C-GG

   Plasmid

   #85092

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Glycine 108 to Cysteine

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Danio rerio cryaa V62T

    Plasmid

    #47630

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Expression

    Bacterial

    Mutation

    Changed Valine 62 to Threonine

    Promoter

    T7

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Danio rerio cryaa T147V

    Plasmid

    #47628

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Expression

    Bacterial

    Mutation

    Changed Theronine 147 to Valine

    Promoter

    T7

    Available Since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Danio rerio cryaa C143S

    Plasmid

    #47619

    Purpose

    Recombinant protein production

    Depositor

    Mason Posner

    Article

    Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

    Insert

    Alpha A-crystallin (cryaa Zebrafish)
    

    Expression

    Bacterial

    Mutation

    Cysteine 143 to Serine

    Promoter

    T7

    Available Since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. EcoFlex MoClo Kit

    Plasmid Kit

    #1000000080

    Purpose

    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design

    Depositor

    Paul Freemont

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

14. p300 KAT LIC 1M

    Plasmid

    #233587

    Purpose

    Bacterial expression of p300 KAT enzyme

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300 KAT (EP300 Human)
    

    Tags

    6xHis MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP1E3D1

    Plasmid

    #20066

    Purpose

    Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

    Insert

    MSP1E3D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeats of …

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. RC(WW)-LgBit

    Plasmid

    #208931

    Purpose

    Recognition domain for in vitro MAPK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    WW domain of PIN1 (PIN1 Human)
    

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC_N_FEV

    Plasmid

    #167757

    Purpose

    Expresses FEV in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    FEV (FEV Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-SNAP-PI Probe

    Plasmid

    #211507

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BcPI-PLC-ANH
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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