We narrowed to 11,765 results for: 110

1. LentiCRISPR v2-sgAMPK alpha2 clone2

   Plasmid

   #162123

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-PGK-dt/CBA-EGFP-R106bT

   Plasmid

   #163921

   Purpose

   Serves as control to pLV-PGK-dt/CBA-EGFP-106bT.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato and EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Added 4-tandem reverse orientation-mir-106b-targe…

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPR v2-sgAMPK alpha2 clone3

   Plasmid

   #162124

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBTetBl-CLIP-γ-tubulin

   Plasmid

   #136848

   Purpose

   Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-TUBG (TUBG1 Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

   Plasmid

   #136656

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:MTH17CL396 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6_sgRNA_CAG_hSt1Cas9_TH1477

   Plasmid

   #136655

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9:TH1477 Chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6_sgRNA_CAG_hSt1Cas9_LMG18311

   Plasmid

   #136653

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:LMG18311 chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and hU6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPC-Flag-SOCS1-Y80D

   Plasmid

   #129516

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Aspartic acid

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPC-Flag-SOCS1-Y80F

   Plasmid

   #129517

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Phenylalanine

   Promoter

   cmv

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-INTS3-C

    Plasmid

    #128642

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 C-termini (INTS3 Human)
    

    Tags

    His tag on N and C-terminal

    Expression

    Bacterial

    Mutation

    INTS3 C-termini (514–1042 aa)

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 289aa Met133, 138, 186Ileu ELL2

    Plasmid

    #127272

    Purpose

    Expresses 289aa human ELL2 with Met133, 138, 186Ileu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
    

    Expression

    Mammalian

    Mutation

    Met133, 138, 186 Ileu, cut with XbaI blunt fillin

    Promoter

    pEF1a

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. frameshift ELL2

    Plasmid

    #127257

    Purpose

    For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    XhoI site between the 1st &2nd Met was filled…

    Promoter

    T7

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 564aa ELL2 bluescript

    Plasmid

    #127267

    Purpose

    For in vitro translation of human ELL2 lacking COOH region

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Addgene Plasmid 127256--SphI site filled in, then…

    Promoter

    T7

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. gh145

    Plasmid

    #106804

    Purpose

    expression of gRNA targeting FKRP

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FKRP (FKRP Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. gh67

    Plasmid

    #106738

    Purpose

    expression of gRNA targeting B3GNT1 (B4GAT1)

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GNT1 (B4GAT1) (B4GAT1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TLK2 gRNA (BRDN0001146448)

    Plasmid

    #75615

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TLK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TLK2 (TLK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TLK2 gRNA (BRDN0001147069)

    Plasmid

    #75616

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TLK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TLK2 (TLK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TLK2 gRNA (BRDN0001147939)

    Plasmid

    #75617

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TLK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TLK2 (TLK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF1-ADAM29 (E111K)

    Plasmid

    #31144

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Glutamic acid 111 changed to Lysine

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

20. mCherry-VPS13C-Cter

    Plasmid

    #232866

    Purpose

    Expresses the C-terminal fragments of VPS13C in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    the C-terminal fragments of VPS13C (VPS13C Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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