We narrowed to 5,563 results for: pCas

1. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. UAS-dsarm(E)

   Plasmid

   #187870

   Purpose

   Gal4/UAS expression of dSarm(isoE)

   Depositor

   Lukas Neukomm

   Article

   Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

   Insert

   dSarm isoform E (Sarm Fly)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMonAID_dCas9-PmCDA_Hyg_ALS

   Plasmid

   #91692

   Purpose

   Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A and H840A for dead Cas9

   Promoter

   2x35S

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-ACBEmax

   Plasmid

   #139106

   Purpose

   Mammalian ACBEmax expression vector (pSI-632)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAd-Track Flag GCN5 Y621A/P622A

   Plasmid

   #14425

   Depositor

   Pere Puigserver

   Article

   Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

   Insert

   GCN5 Y621A/P622A (KAT2A Human)
   

   Use

   Adenoviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Y621A, P622A. Catalytically inactive acetyltransf…

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

   Plasmid

   #134612

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 375 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyCas9 PE2*-3HA

   Plasmid

   #169854

   Purpose

   Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-star-M-MLV-3HA
   

   Use

   Tags

   3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPmacroH2A.1-GS3

   Plasmid

   #204759

   Purpose

   Expression of Cas9 and human macroH2A.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   macroH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Use

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CXN-BCL-6 ZFΔ (pCXN2-BCL6-ZFdel)

    Plasmid

    #40347

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    BCL-6 ZFΔ (BCL6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains aa1-504; ZF domain deleted (see note bel…

    Promoter

    pCAG

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pTAL_RFP

    Plasmid

    #43859

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330 NL-FKBP-Cas9

    Plasmid

    #162723

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDP012

    Plasmid

    #101167

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTAL_GFP

    Plasmid

    #43858

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSOMA-T679A-NLS

    Plasmid

    #118938

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173860

    Purpose

    Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    mCherry-PHR-iSH-T2A-CIBNcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 N-FKBP-Cas9

    Plasmid

    #162727

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. VIPR1

    Plasmid

    #51865

    Purpose

    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    VIPR1 (VIPR1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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