We narrowed to 5,839 results for: pCas

1. pX458-Ef1a-Cas9-H2B-GFP

   Plasmid

   #159654

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p35S:Citrine-TurboID-3xHA

   Plasmid

   #199243

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID
   

   Tags

   3xHA and TurboID-3xHA

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBQ:Citrine-TurboID-3xMyc

   Plasmid

   #199244

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID-3xMyc
   

   Tags

   TurboID-3xMyc

   Expression

   Plant

   Promoter

   Arabidopsis UBQ10 promoter with intron

   Available Since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Td-CGBE

   Plasmid

   #196599

   Purpose

   Mammalian expression of Td-CGBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L); S. pyogenes Cas9 (D10A)

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. YEE-BE4max

   Plasmid

   #138157

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YEE)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-NRCH-PE2max-BSD

   Plasmid

   #191103

   Purpose

   Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   NRCH-PEmax
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed R221K, N394K, H840A from SpCas9-NRCH

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. FZ-NbPDS

   Plasmid

   #184268

   Purpose

   pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.

   Depositor

   Feng Qu

   Article

   Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

   Insert

   CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
   

   Expression

   Plant

   Mutation

   The protease processing site between MP and L-CP …

   Promoter

   CaMV 35S with duplicated enhancer

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AgDD

   Plasmid

   #78289

   Purpose

   create protein aggregates in living cells

   Depositor

   Thomas Wandless

   Article

   Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

   Insert

   NES-L106P-sfGFP (FKBP1A Human)
   

   Tags

   MLALKLAGLDI

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-Amp_CRISPIE donor B5

    Plasmid

    #172846

    Purpose

    CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. px459-TDP-43 sgRNA1

    Plasmid

    #133762

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cre-responsive Cas9 expressing transposon library vector

    Plasmid

    #164138

    Purpose

    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, EF-1a

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330 _CRISPIE donor B8

    Plasmid

    #172849

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MBP-TSF-TEVcs-ULK1K46I

    Plasmid

    #171417

    Purpose

    For the purifcation of human ULK1 kinase dead complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Expression

    Mammalian

    Mutation

    kinase dead

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FL MALT1 WT in pET29b-Strep

    Plasmid

    #48968

    Purpose

    Expresses Full length wild type MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Tags

    His and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

17. IL1RN gRNA4

    Plasmid

    #113133

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRvCAHS3-CAHS3-mEGFP

    Plasmid

    #205006

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    RvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRvSAHS1-SAHS1-mEGFP

    Plasmid

    #205007

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    RvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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