We narrowed to 595 results for: pFAST

1. pFB-EPCP5

   Plasmid

   #228879

   Purpose

   Expression of Z-tagged LRRK2 KW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Use

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1846

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB-GST-IRP2

   Plasmid

   #226621

   Purpose

   Expresses human IRP2 in insect cells

   Depositor

   Ning Zheng

   Article

   Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

   Insert

   IRP2 (IREB2 Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac1-BacMam-4x-PyOtR

   Plasmid

   #217364

   Purpose

   Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   4 copies of pyrrolysyl-tRNA mutant PyOtR
   

   Use

   Baculoviral

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. S+Q111R

   Plasmid

   #167173

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+Q111R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed Q at 111 to R

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. S+N122D

   Plasmid

   #167174

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+N122D
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed N at 111 to D

   Promoter

   P10 and PH

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mWasabi-ADAP1

   Plasmid

   #163906

   Purpose

   Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Use

   Tags

   mWasabi fluorescent tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB1.HMBP.PrS.dEZH2

   Plasmid

   #220241

   Purpose

   Expression of an EZH2 catalytic site mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   dEZH2 (EZH2 Human)
   

   Use

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   Y731F

   Promoter

   Polyhedrin

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB1.HMBP.PrS.JARID2

   Plasmid

   #125166

   Purpose

   Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   JARID2 (JARID2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.EPOP

   Plasmid

   #125170

   Purpose

   Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   EPOP (EPOP Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. R-Q111R-N122D

    Plasmid

    #167178

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-R-Q111R-N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed R at 111 to Q and changed D at 122 to N

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid

    #224708

    Purpose

    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 chromodomain truncation (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB1.HMBP.PrS.CBX8-KR21A

    Plasmid

    #224709

    Purpose

    Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 KR21A mt (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFB1.HMBP.PrS.CBX8

    Plasmid

    #224707

    Purpose

    Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    HMBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB1.HMBP.PrS.EZH2-mt1

    Plasmid

    #220239

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    R27A, R31A, F165A

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. S+Q111R+N122D

    Plasmid

    #167175

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+Q111R+N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R and changed N at 122 to D

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S+10subs

    Plasmid

    #167176

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+10R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed A at 112 to T; E at 116 to D; I at 135 to…

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. S+12subs

    Plasmid

    #167177

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+12R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R; A at 112 to T; E at 116to …

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFB1.HMBP.PrS.RBBP4

    Plasmid

    #125164

    Purpose

    Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Histone-binding protein RBBP4 (RBBP4 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFB1.HMBP.PrS.EZH2

    Plasmid

    #125161

    Purpose

    Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Baculovirus expression vector

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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