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We narrowed to 18,241 results for: multi

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Showing: 5221 - 5240 of 18241 results

  1. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    99214-plasmid-map-sequence-id-192369

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  4. pCali2

    Plasmid
    #86119
    Purpose
    Multiplex temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Publication
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA36
    mWasabi
    TcI
    mCherry
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T and D341A, P60L, D135V, K187R, K202I, L208Q
    Promoter
    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI
    Available since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86119-plasmid-map-sequence-id-173197

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  5. pAC-94N

    Plasmid
    #53281
    Purpose
    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase
    Depositor
    Francis X Cunningham Jr
    Publication
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    53281-plasmid-map-sequence-id-82992

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  6. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Publication
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

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  8. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Publication
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  16. pCAGGS-mTagBFP2-T2A-iCre

    Plasmid
    #113837
    Purpose
    Monomeric TagBFP2 and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCre
    Expression
    Mammalian
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113837-plasmid-map-sequence-id-222246

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  20. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75154
    Purpose
    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75154-plasmid-map-sequence-id-142108

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Showing: 5221 - 5240 of 18241 results