We narrowed to 10,183 results for: yeast

1. pAC-BETAipi-ispA/dxs

   Plasmid

   #178302

   Purpose

   Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Inserts

   ispA
   dxs
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193315

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Tags

   4FLAG

   Expression

   Bacterial

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWS5399-CRISPRai-ZeoR

   Plasmid

   #182838

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWS5321-CRISPRai-Markerless

   Plasmid

   #182828

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - markerless

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS143

   Plasmid

   #122377

   Purpose

   CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYES2-HTH-HHT2

   Plasmid

   #86482

   Purpose

   yeast expression of HHT2 with His10-TEV-HA tag

   Depositor

   Akash Gunjan

   Article

   Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

   Insert

   HHT2 (HHT2 Budding Yeast)
   

   Tags

   HIS10-TEV-HA (HTH)

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. p404-MTS-mCherry-GFP1-10

   Plasmid

   #91958

   Purpose

   Expresses mCherry-GFP1-10 in yeast cell mitochondria

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   MTS-mCherry-GFP1-10
   

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   June 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWS5398-CRISPRai-HygR

   Plasmid

   #182837

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWS5390-CRISPRai-URA3

   Plasmid

   #182829

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PP244

    Plasmid

    #78978

    Purpose

    Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Expression

    Yeast

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PP78

    Plasmid

    #78948

    Purpose

    Supplemental Figure 3. Integration Site 5.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    Integration Site 5
    

    Expression

    Yeast

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS406-PHO5-GFP-hFAPP1 PH domain

    Plasmid

    #58723

    Purpose

    Expresses GFP-Tagged human FAPP1 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Promoter

    PHO5

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pWS5396-CRISPRai-KanR

    Plasmid

    #182835

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-trAtCCD8(pYL735)

    Plasmid

    #178286

    Purpose

    Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    trAtCCD8
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJO629

    Plasmid

    #104342

    Purpose

    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

    Insert

    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    

    Tags

    3xFLAG

    Expression

    Yeast

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYR69 (X-4:4xUAS-SSA1p-365:yEGFP3-CYC1t)

    Plasmid

    #167355

    Purpose

    Integrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    4xUAS-SSA1p-365:yEGFP3-CYC1t
    

    Use

    Unspecified

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Plasmid

    #58440

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
    

    Expression

    Yeast

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. YIPlac204TKC-E2-Crimson-HDEL

    Plasmid

    #38771

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Tags

    Linker peptide and yeast ER retention signal sequ…

    Expression

    Yeast

    Promoter

    TPI1

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Plasmid

    #58439

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
    

    Expression

    Yeast

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9v2

    Plasmid

    #103140

    Purpose

    TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    dCas9-VPR
    

    Expression

    Yeast

    Mutation

    Removed scaffold gRNA from original plasmid

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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