We narrowed to 5,525 results for: golden gate
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pNK189
Plasmid#219743PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiensDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK4169
Plasmid#219744PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastorisDepositorInsertmutant of fungal hispidin-3-hydroxylase
UseSynthetic BiologyMutationD37E, V181I, S323M, M385KAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK845
Plasmid#219746PurposeMoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiensDepositorInsertmutant of fungal luciferase
UseLuciferase and Synthetic BiologyMutationI3S, N4T, F11L, I63T, T99P, T192S, A199PAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK291
Plasmid#219748PurposeDVK_AF CIDAR vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of CMV promoter, for mammalian expressionDepositorInsertpCMV - nnH3H_v2 - tSV40
ExpressionMammalianAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH101
Plasmid#215772PurposeNBU2 backbone with LacI(YQR) and P(O1)_nanolucDepositorInsertsLacI(YQR)
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBT1311 and P(O1)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH102
Plasmid#215773PurposeNBU2 backbone with LacI(TAN) and P(tta)_nanolucDepositorInsertsLacI(TAN)
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBt1311 and P(tta)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH103
Plasmid#215774PurposeNBU2 backbone with LacI(KSL) and P(agg)_nanolucDepositorInsertsLacI(KSL)
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBt1311 and P(agg)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH104
Plasmid#215775PurposeNBU2 backbone with LacI(HQN) and P(ttg)_nanolucDepositorInsertsLacI(HQN)
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBt1311 and P(ttg)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH105
Plasmid#215776PurposeNBU2 backbone with LacI(GKR) and P(gac)_nanolucDepositorInsertsLacI(GKR)
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBt1311 and P(gac)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH106
Plasmid#215777PurposeNBU2 backbone with RbsR(YQR) and P(O1)_nanolucDepositorInsertsRbsR(YQR)
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBt1311 and P(O1)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH116
Plasmid#215779PurposeNBU2 backbone with anti-RbsR(YQR) and P(O1)_nanolucDepositorInsertsanti-RbsR(YQR)
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBt1311 and P(O1)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBH501
Plasmid#215780PurposeNBU1 backbone with constitutive dCas9DepositorInsertdCas9
UseSynthetic BiologyExpressionBacterialPromoterP1Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-FXa-cleavableELP
Plasmid#219403PurposeFactor Xa-responsive elastin-like polypeptide (ELP)DepositorInsertFXa-cleavableELP
Tags6xHisExpressionBacterialAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EK-cleavableELP
Plasmid#219404PurposeEnterokinase-responsive elastin-like polypeptide (ELP)DepositorInsertEK-cleavableELP
Tags6xHisExpressionBacterialAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ1-g1g2-K2
Plasmid#218217PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatibleDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLCHKOv3-mClover-intergenic_2-As
Plasmid#218814PurposeLentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectivelyDepositorInsertintergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mClover-intergenic_3-As
Plasmid#218815PurposeLentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectivelyDepositorInsertintergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCHKOv3-mClover-intergenic_4-As
Plasmid#218816PurposeLentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectivelyDepositorInsertintergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-spacer-H1
Plasmid#218399Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
ExpressionMammalianPromotertetracycline-responsive promoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only
