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We narrowed to 795 results for: KO

Showing: 521 - 540 of 795 results

  2. pαH-S-GSAS-B.1.351.v4

    Plasmid
    #173788
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 4
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 4) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    173788-plasmid-map-sequence-id-352110

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  7. pαH-S-GSAS-B.1.1.7

    Plasmid
    #171747
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171747-plasmid-map-sequence-id-337765

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  8. pαH-S-GSAS-B.1.351.v2

    Plasmid
    #173786
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    173786-plasmid-map-sequence-id-340773

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  9. pαH-S-GSAS-B.1.351

    Plasmid
    #171749
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171749-plasmid-map-sequence-id-337764

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  10. pαH-S-GSAS-D614G-K417N-E484K-N501Y

    Plasmid
    #171750
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501Y
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171750-plasmid-map-sequence-id-337761

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  11. pαH-S-GSAS-D614G-deltaFV

    Plasmid
    #171743
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    171743-plasmid-map-sequence-id-337714

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  12. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  13. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  14. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-1

    Plasmid
    #237890
    Purpose
    Cre-KO AAV vector#1 containing SaCas9 and its sgRNA targeting Cre
    Depositor
    Rui Lin
    Insert
    sgRNA-Cre
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    237890-plasmid-map-sequence-id-481798

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  15. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-2

    Plasmid
    #237891
    Purpose
    Cre-KO AAV vector#2 containing SaCas9 and its sgRNA targeting Cre
    Depositor
    Rui Lin
    Insert
    sgRNA-Cre
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    237891-plasmid-map-sequence-id-481799

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  16. pX601-AAV-CMV-NLS-SaCas9-NLS-3xHA-bGHpA;U6-Cre-3

    Plasmid
    #237892
    Purpose
    Cre-KO AAV vector#3 containing SaCas9 and its sgRNA targeting Cre
    Depositor
    Rui Lin
    Insert
    sgRNA-Cre
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    237892-plasmid-map-sequence-id-481792

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  17. pαH-S-GSAS-2P-Omicron-BA.5

    Plasmid
    #213828
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/Omicron-2P-BA5 (S )
    Use
    Tags
    HRV 3C cleavage site (before tags) 8X His tag 2X…
    Expression
    Mammalian
    Mutation
    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213828-plasmid-map-sequence-id-423246

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  18. pcDNA3.1/hFGFR3-K650M-V5

    Plasmid
    #214909
    Purpose
    expression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)
    Depositor
    Pavel Krejčí
    Article
    Kunova Bosakova et al Proc Natl Acad Sci U S A. 2019 Feb 19. p
    Insert
    FGFR3 (FGFR3 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    K650M substitution
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214909-plasmid-map-sequence-id-426045

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  19. pαH-rS2d

    Plasmid
    #183516
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    rS2d (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183516-plasmid-map-sequence-id-362651

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  20. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Drew Endy
    Available since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 521 - 540 of 795 results