We narrowed to 6,957 results for: mag

1. tetO-HEY2

   Plasmid

   #170691

   Purpose

   doxycycline-inducible overexpression of HEY2

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   HEY2 – hes related family bHLH transcription factor with YRPW motif 2 (HEY2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

   Plasmid

   #196885

   Purpose

   Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
   

   Use

   Cre/Lox; Dre/rox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR-53BP1 1-1972 28A

   Plasmid

   #53447

   Purpose

   Recombinational donor/master vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   I914T mutation; All 28 SQ/TQ motifs in N-term of …

   Promoter

   Available since

   Dec. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Desmoplakin I acceptor only control (F40-based tension sensor)

   Plasmid

   #119189

   Purpose

   The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-Myc-FBW7 Delta F-Box

   Plasmid

   #197452

   Purpose

   The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW7 Fbox deleted.

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBactin-td-mCherry-Cdk5rap2-CTD

   Plasmid

   #196854

   Purpose

   Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220610

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Desmoplakin I affinity mutant (F40-based tension sensor)

   Plasmid

   #119190

   Purpose

   The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Beta-2-adrenergic receptor-mCerulean

   Plasmid

   #66957

   Purpose

   Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   Beta-2-adrenergic receptor-mCerulean (ADRB2 Human, Aequorea victoria)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ai14-HITI

    Plasmid

    #87117

    Purpose

    AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2-shATRX

    Plasmid

    #124258

    Purpose

    Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shATRX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid

    #197506

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid

    #196853

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57-mCherry-2A-BRD4 Iso AdelCTD

    Plasmid

    #137722

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated at amino acid 1328

    Promoter

    Tight TRE promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-MCU-mCerulean

    Plasmid

    #185809

    Purpose

    Human mitochondrial calcium uniporter with C-terminus mCerulean tag

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    Mitochondrial calcium uniporter (MCU Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA

    Plasmid

    #196896

    Purpose

    Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    N-Cadherin-2xHA (Cdh1 Mouse)
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-ARAP3(R307,8A)

    Plasmid

    #39486

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3(R307,8A) (ARAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R307,8A = PH domain mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid

    #137723

    Purpose

    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

    Depositor

    Scott Floyd

    Article

    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

    Insert

    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 600-682

    Promoter

    Tight TRE promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221-ACE2 receptor 19-615

    Plasmid

    #167012

    Purpose

    Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

    Depositor

    Spyros Petrakis

    Article

    Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids 19-615, No stop codon

    Promoter

    Available since

    March 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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