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We narrowed to 6,957 results for: mag

Showing: 5781 - 5800 of 6957 results

  1. pCAGGS-SMSr-TEV-Twin-Strep

    Plasmid
    #202530
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SAMD8 (SAMD8 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TGA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202530-plasmid-map-sequence-id-406370

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  2. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

    Plasmid
    #197454
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 Delta-WD40 propeller (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197454-plasmid-map-sequence-id-394916

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  3. pDEST-Myc-FBW7 WD40 (All deleted)

    Plasmid
    #197455
    Purpose
    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FBW& dimerization domain deleted.
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197455-plasmid-map-sequence-id-394905

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  5. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid
    #196894
    Purpose
    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    N-gTBD-mOrange2 (NEDD1 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196894-plasmid-map-sequence-id-392229

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  6. pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3

    Plasmid
    #196895
    Purpose
    Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    2xHA-CAMSAP3 (Camsap3 Mouse)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196895-plasmid-map-sequence-id-392230

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  7. pBactin-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196855
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196855-plasmid-map-sequence-id-391813

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  8. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid
    #196856
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lacking the centrosome-targeting domain (CTD)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196856-plasmid-map-sequence-id-391815

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  9. pInducer 21-3xFlag-hMEFV-F479L

    Plasmid
    #195477
    Purpose
    pInducer21 plasmid containing the human MEFV gene with the F479L mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed phenylalanine 479 to leucine
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195477-plasmid-map-sequence-id-386697

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  10. pInducer 21-3xFlag-hMEFV-ΔPYD

    Plasmid
    #195478
    Purpose
    pInducer21 plasmid containing the human MEFV gene with a deletion of residues 1 - 92 (the resulting pyrin protein lacks the PYD domain) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    deletion of residues 1-92 of pyrin (the PYD domai…
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195478-plasmid-map-sequence-id-386693

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  11. pInducer21-3xFlag-hMEFV-Q426R

    Plasmid
    #195475
    Purpose
    pInducer21 plasmid containing the human MEFV gene with the Q426R mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed glutamine 426 to arginine
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195475-plasmid-map-sequence-id-386689

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  12. pInducer 21-3xFlag-hMEFV-S580X

    Plasmid
    #195474
    Purpose
    pInducer21 plasmid containing the human MEFV gene with the S580X mutation (stop codon leading to the truncation of the B30.2 domain of the pyrin protein) with a 3xFlag tag at the N-terminus
    Depositor
    Thomas Henry
    Article
    Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d
    Insert
    MEFV (MEFV Human)
    Use
    Lentiviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed S580 to a stop codon
    Promoter
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    195474-plasmid-map-sequence-id-386691

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  13. Desmoplakin II donor only control (FL-based no force control)

    Plasmid
    #118722
    Purpose
    The donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118722-plasmid-map-sequence-id-230466

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  14. Desmoplakin II acceptor only control (F40-based no force control)

    Plasmid
    #118720
    Purpose
    The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y67G mutation in YPet(sh…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118720-plasmid-map-sequence-id-230457

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  15. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid
    #192786
    Purpose
    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR1 (IFNGR1 Human)
    Use
    Tags
    Ig k-chain leader sequence and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192786-plasmid-map-sequence-id-379664

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  16. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid
    #175181
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175181-plasmid-map-sequence-id-345963

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  17. pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt

    Plasmid
    #175184
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLEX iGluSnFR3 v82.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175184-plasmid-map-sequence-id-345962

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  18. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

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  19. pSems-leader-mXFPe-IFNGR2(30-337)

    Plasmid
    #192787
    Purpose
    Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR2 (IFNGR2 Human)
    Use
    Tags
    Ig k-chain leader sequence and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192787-plasmid-map-sequence-id-379660

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  20. AAV-pCbh-ABE8e-nSpCas9(D10A/A61R)[N-term] (CA19)

    Plasmid
    #197510
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A/A61R) (to be used with SpRY C-term constructs)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A/A61R)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Expression
    Mutation
    ABE8e mutations in TadA and nSpRY(D10A/A61R)
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197510-plasmid-map-sequence-id-386692

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Showing: 5781 - 5800 of 6957 results