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pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-TAL-NC2

Plasmid

#43856

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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Munc18cF119E-pDEST27

Plasmid

#85625

Purpose

GST tagged Munc18c with a F119E mutation

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Mutation

F119E

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Expression

Bacterial

Promoter

LacUV5

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-FRB-HA no-pA

Plasmid

#82595

Purpose

3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-FRB-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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LEPG_shSik3.3974

Plasmid

#125855

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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LEPG_shSik3.308

Plasmid

#125878

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRSV-p65T305A,S276A

Plasmid

#106456

Purpose

NF-kB p65, kinase sites 276,305 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase sites 276,305 mutated (RELA Human)

Expression

Mammalian

Mutation

kinase sites inactivated

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-ZO1-C-14

Plasmid

#55518

Purpose

Localization: Tight Junctions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

ZO1 (TJP1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
HCKan-P-TEF2

Plasmid

#65337

Purpose

construction of TEF2 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 promoter

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMT mir-1-1 reporter cel-mir-40

Plasmid

#46739

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-40

Use

RNAi

Tags

His and hsa-mir-1-1

Expression

Insect

Promoter

Metallothionein (MT)

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtYB

Plasmid

#65346

Purpose

construction of ycrtYB gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtYB

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtE

Plasmid

#65344

Purpose

construction of ycrtE gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtE

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-O-ycrtI

Plasmid

#65345

Purpose

construction of ycrtI gene in HCKan-O receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ycrtI

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseH

Plasmid

#89725

Purpose

Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseH (pseH Campylobacter jejuni)

Tags

2xHis Gln 5xHis and TEV

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSY075

Plasmid

#110067

Purpose

Bacterial expression of L2

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L2

Expression

Bacterial

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSL14 - Prp8 2142–2398

Plasmid

#90264

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

PRP8 (PRP8 Budding Yeast)

Tags

N-terminal 6xHis tag

Expression

Bacterial

Mutation

only Jab1 domain (aa 2143-2398)

Promoter

T7

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDS1U-PKS12

Plasmid

#70150

Purpose

Fusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

PKS12 (PKS12 Fusarium graminearum)

Expression

Yeast

Promoter

CUP1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLSLZ

Plasmid

#51495

Purpose

pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
3kb filler sequence

Use

Plant t-dna plasmid

Tags

SV40 NLS

Promoter

virion-sense LIR and 2x35S

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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