We narrowed to 38 results for: lic

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Learn how the 2017 Addgene-Seeding Labs Plasmid Grant Awardees will be using plasmids in their research.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein 3D structure and crystallography.

Protocol for Ligation Independent Cloning

A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more.

We're updating our Hold for Publication policy. Read about how these changes will help streamline our workflows and get your plasmids online faster!

Learn about click biology and how you can use it to advance your experiments and explore biological phenomenon.

Learn about different molecular cloning techniques, focusing on seven common cloning methods.

Dora Farkas, career coach for scientists, discusses how to identify difficult supervisors and outlines 7 strategies to resolve conflicts.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.

Ghanaian researcher, Kwabena Duedu discusses his work in public health, synthetic biology, and microbiology.

List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.

Synthetic origins of replication, or ORIs, from the Chappell Lab have improved plasmid compatibility and copy number control compared to natural ORIs.

Applying for a PhD post-COVID? Our guest blogger walks you through how to use the virtual/hybrid options to your advantage!

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Learn more about NanoBiT technology and explore examples of HiBiT-tagged fusion proteins in action.

Plasmids can be under either stringent regulation of replication or relaxed regulation of replication. In this blog post, we explain how stringent regulation works.

If you present data to others, you have a valuable transferable skill: public speaking!

An overview of viral applications spanning from basic research science to clinical therapies.