Ligation Independent Cloning
Type
Protocol
... as an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol...Protocols Overview Ligation Independent Cloning (LIC) obviates the need for the time-consuming ligation... through the transformation/replication process. LIC employs long overhangs to form a stable association... during the replication cycle. Empty vectors for LIC typically employ type II restriction enzymes (e.g...in the example shown here. The manufacturer of an LIC-specific vector will provide the homologous sequence...Protocol Step 1: Design Your Primers Primer design for LIC is often as simple as using the backbone manufacturer's... protocol . Search Addgene's collection of empty LIC cloning vectors...