We narrowed to 6,252 results for: nls

1. pSCL.94

   Plasmid

   #184985

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker2-Cas9
   

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.006

   Plasmid

   #184970

   Purpose

   Express -Eco1 RT in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Eco1 RT
   

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.005

   Plasmid

   #184971

   Purpose

   Express Cas9 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Cas9
   

   Tags

   SV40NLS

   Expression

   Yeast

   Promoter

   Gal1-10

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCL.71

   Plasmid

   #184983

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker1-Cas9
   

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS538

   Plasmid

   #188881

   Purpose

   Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Cas9
   

   Use

   Gateway entry vector

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL30

   Plasmid

   #174064

   Purpose

   Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.

   Depositor

   Albert Keung

   Article

   Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

   Insert

   pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   pGal1

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH3971

   Plasmid

   #177736

   Purpose

   Prgef-1 GCaMP6::3xNLS::mNeptune

   Depositor

   Mei Zhen

   Article

   Susoy et al Cell. 2021 Sep 30;184(20):5122-5137.e17.

   Insert

   GCaMP
   

   Tags

   mNeptune

   Expression

   Worm

   Promoter

   Prgef-1

   Available Since

   Dec. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pJL15

   Plasmid

   #174069

   Purpose

   Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

   Depositor

   Albert Keung

   Article

   Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

   Insert

   pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   pGal1

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJF152_Phsp_Cas9

   Plasmid

   #163862

   Purpose

   heat shock-induced expression of Cas9 in C. elegans

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Insert

   Cas9, C. elegans codon optimized with intron and SV40_NLS
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   Phsp-16.48

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL-GFPGO2(NGC)-PGK-Neo

    Plasmid

    #136893

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO2(NGC)
    

    Use

    Lentiviral

    Tags

    2xNLS

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL-GFPGO2(NGT)-PGK-Neo

    Plasmid

    #136892

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO2(NGT)
    

    Use

    Lentiviral

    Tags

    2xNLS

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK4a_026

    Plasmid

    #123838

    Purpose

    Encodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    HA::2xNLS::ABI
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK4a_027

    Plasmid

    #123839

    Purpose

    Encodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    HA::2xNLS::GAI
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK1_015

    Plasmid

    #123678

    Purpose

    Encodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-2xHS4-LoxP
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDA186

    Plasmid

    #74250

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Expression

    Bacterial and Yeast

    Promoter

    pGPD1

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDA171

    Plasmid

    #74251

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Tags

    Venus

    Expression

    Bacterial and Yeast

    Promoter

    pSTL1

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK1334

    Plasmid

    #127965

    Purpose

    sgRNA expression vector compatible with CROP-Seq and imaging screens

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MCP-M-MLV RT∆RNase H

    Plasmid

    #213749

    Purpose

    For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA012

    Plasmid

    #215974

    Purpose

    Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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