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pEBB-II-I-GFP gamma isoform

Plasmid

#22148

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pUAS-hActin-luc2

Plasmid

#48081

Purpose

Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Luciferase

Expression

Mammalian

Mutation

removed hsp70 promoter, inserted amplified 615 bp…

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pLEX_970_puro_DEST_V5_BFP

Plasmid

#45297

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pBEAST_J23101-amidase

Plasmid

#128135

Purpose

The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

amidase

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo1

Plasmid

#157805

Purpose

Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo1

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713_zhang2.0

Plasmid

#167916

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLMB713

Plasmid

#83785

Purpose

Salicylic acid biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL1329 (salA) containing all of RL1328 (salR)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I alpha

Plasmid

#22154

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pCS2+ Bmi1-cherry-HA

Plasmid

#30516

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

Bmi1 (Bmi1 Mouse)

Use

Xenopus/zebrafish expression

Tags

cherry and triple HA

Expression

Mammalian

Mutation

NO STOP

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pJPF0190

Plasmid

#130268

Purpose

Piggybac vector for the expression of mRuby2-fused human PRKACA

Depositor

Hana El-Samad

Article

Fonseca et al bioRxiv 2020, 747030

Insert

PRKACA (PRKACA Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS2D

Plasmid

#13285

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa150-aa155

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pKMV-VioE

Plasmid

#65351

Purpose

systhesis of vioE gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioE

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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SLF091: Lenti-CaMKIIa-eTsChR-eGFP

Plasmid

#112281

Purpose

For lentiviral or direct expression of eTsChR in excitatory neurons

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

eTsChR

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR macroH2A1.2-C

Plasmid

#30512

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Gateway - donor vector

Mutation

NO STOP

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX_970_puro_DEST_YFP

Plasmid

#45295

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

YFP

Use

Lentiviral

Expression

Mammalian

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
CALNL-RARa-VP16

Plasmid

#185560

Purpose

Cre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

RARa-VP16 (Rara Mouse)

Tags

VP16

Expression

Mammalian

Promoter

CAG

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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mTFP1-CathespinB-6

Plasmid

#55467

Purpose

Localization: Lysosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CathespinB

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only