We narrowed to 16,185 results for: grna

1. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121816

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197025

   Purpose

   Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized RhCas12f1
   

   Expression

   Mammalian

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

   Plasmid

   #86195

   Purpose

   U6 based expression of N meningitidis sgRNA

   Depositor

   Scot Wolfe

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   nmCas9 sgRNA cloning cassete
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

   Plasmid

   #178996

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

   Plasmid

   #178995

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

   Plasmid

   #113041

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

   Plasmid

   #113697

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

   Plasmid

   #113040

   Purpose

   AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128342

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna7 (Chrna7 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

    Plasmid

    #113042

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128338

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna3 (Chrna3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128337

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna2 (Chrna2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128339

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna4 (Chrna4 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128344

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrnb3 (Chrnb3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid

    #242662

    Purpose

    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4

    Depositor

    Benjamin Kleinstiver

    Insert

    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    

    Use

    AAV and CRISPR

    Tags

    BPNLS

    Mutation

    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

    Promoter

    CBh

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid

    #242661

    Purpose

    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4

    Depositor

    Benjamin Kleinstiver

    Insert

    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    

    Use

    AAV and CRISPR

    Tags

    BPNLS

    Mutation

    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

    Promoter

    CBh

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. AgU6.695&557_3xP3-DsRed1-SV40 (Tandem 1_2 gRNAs)

    Plasmid

    #243012

    Purpose

    For cloning two gRNAs in tandem after BspQI digestion under An. gambiae U6.695 and U6.557 promoters.

    Depositor

    George Christophides

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Insect

    Available Since

    Sept. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #239608

    Purpose

    Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    filler-trcr-H1-filler
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231367

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231368

    Purpose

    Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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