We narrowed to 23,204 results for: grna

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pXJ521-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-hU6-gRNA-PB5'IR

Plasmid

#254956

Purpose

AAV piggyBac vector with single gRNA and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Publication

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_sgRNA

Plasmid

#68422

Purpose

Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Gluc sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109314

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

Depositor

William Lagor

Publication

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pYPQ141D-gRNA2.1

Plasmid

#167161

Purpose

Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97308

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(12))-PGKpuroBFP-W

Plasmid

#208569

Purpose

Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX1(13), RUNX3(12) (RUNX1, RUNX3 Human)

Use

Lentiviral

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(RUNX1(13))-hU6gRNA5(RUNX3(13))-PGKpuroBFP-W

Plasmid

#208570

Purpose

Lentiviral vector expressing gRNA targeting human RUNX1 and RUNX3

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX1(13), RUNX3(13) (RUNX1, RUNX3 Human)

Use

Lentiviral

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(21))-PGKpuroBFP-W

Plasmid

#200507

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), ARID1B(21) (ARID1A, ARID1B Human)

Use

Lentiviral

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-W

Plasmid

#200508

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), ARID1B(23) (ARID1A, ARID1B Human)

Use

Lentiviral

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

Plasmid

#200505

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)

Use

Lentiviral

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

pU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pADH99

Plasmid

#90979

Purpose

NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.

Depositor

Aaron Hernday

Publication

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

US_HIS1
FRT
pENO1
C. albicans Cas9
pMAL2
FLP
NAT 1 of 2

Use

CRISPR

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBbdCas9S(-10TC)_Psyn-sgRNA500

Plasmid

#149583

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Neg. Ctrl_sgRNA

Plasmid

#111298

Purpose

CRISPR KO murine neg. ctrl.

Depositor

Vamsi Mootha

Publication

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

NegCtrl sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaBS-sgRNA500

Plasmid

#149615

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaBS

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA2

Plasmid

#134634

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA2 (UFM1 Human)

Expression

Mammalian

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA1

Plasmid

#134633

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA1 (UFM1 Human)

Expression

Mammalian

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p425_Cas9_gRNA_LEU_1014a

Plasmid

#87407

Purpose

p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.

Depositor

Aindrila Mukhopadhyay

Publication

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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