We narrowed to 8,829 results for: sgRNA

1. TLCV2 - sgAP2S1 #2

   Plasmid

   #202757

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting AP2S1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2 - sgAP2M1

   Plasmid

   #202758

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting AP2M1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2 - sgAP2S1 #1

   Plasmid

   #202756

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting AP2S1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. psBIG1a ActBT2ATetP2AP - mAGH

   Plasmid

   #229973

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - mAGH
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. psBIG1a ActBT2ATetP2AP - H

   Plasmid

   #229972

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2ATetP2AP - H
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. psBIG1a ActBT2AmcherryP2AP -TagBFPH

   Plasmid

   #229971

   Purpose

   Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBT2AmcherryP2AP -TagBFPH
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEVA47_dCas9-GFP_NOT_i1

   Plasmid

   #231416

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-dSa VP64 Afp

   Plasmid

   #99697

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Afp promoter, vector allows for activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Mutation

   dead Cas9

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpy

   Plasmid

   #220184

   Purpose

   sgRNA expression vector - promoterless backbone

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   promoterless backbone
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   promoterless

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpyA

    Plasmid

    #220185

    Purpose

    sgRNA expression vector - pEM7 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pEM7

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpyC

    Plasmid

    #220187

    Purpose

    sgRNA expression vector - pBG42 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pBG42

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpyD

    Plasmid

    #220188

    Purpose

    sgRNA expression vector - pBG28 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG28 promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pBG28

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBH504

    Plasmid

    #215782

    Purpose

    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene

    Depositor

    Corey Wilson

    Article

    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

    Inserts

    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Bt1311, P(O1), and P1

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PlayBack-U6-Bamh1

    Plasmid

    #203298

    Purpose

    For expression of sgRNAs in a PlayBack Vector with Bamh1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PlayBack-U6-Bgl11

    Plasmid

    #203299

    Purpose

    For expression of sgRNAs in a PlayBack Vector with BglII as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PlayBack-U6-Not1

    Plasmid

    #203301

    Purpose

    For expression of sgRNAs in a PlayBack Vector with NotI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSUI-SynP-sfGFP

    Plasmid

    #154306

    Purpose

    Expression of sfGFP under Synthetic promoter (SynP) and cloning of the sgRNA with simple screening of positive clone by fluorescence

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAN-PA5-RFP

    Plasmid

    #62251

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Expression

    Bacterial

    Promoter

    pA5

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCut plasmid toolkit

    Plasmid Kit

    #1000000118

    Purpose

    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.

    Depositor

    Aindrila Mukhopadhyay

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pDGE Dicot Genome Editing Kit

    Plasmid Kit

    #1000000084

    Purpose

    Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of "genome editing recipient" and "sgRNA shuttle" vectors.

    Depositor

    Johannes Stuttmann

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only