We narrowed to 8,677 results for: sgRNA

1. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

   Plasmid

   #114729

   Purpose

   3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

   Depositor

   Martine Roussel

   Article

   Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

   Insert

   sgRNA M5, sgRNA M7, sgRNA M9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74009

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5x UAS
   Cas9
   T2A
   GFP
   U6 promoter
   

   Use

   CRISPR

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

   Plasmid

   #164266

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

   Depositor

   Nikolaus Rajewsky

   Article

   Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6 promoter from W05B2.8

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Promoter

   EFS, No promoter, and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-sgRNA

   Plasmid

   #71409

   Purpose

   There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

   Depositor

   David Sabatini, Eric Lander

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATG16L1 sgRNA

   Plasmid

   #207563

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   GCAGCAAGTGACATGTCGTC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   David Sabatini, Eric Lander

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF142_U6-sgRNA

    Plasmid

    #225960

    Purpose

    U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    U6-sgRNA (recipient)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcU6_3 sgRNA

    Plasmid

    #92359

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    chick U6.3

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAKN2_J23119-sgRNA

    Plasmid

    #108552

    Purpose

    Bacterial sgRNA expression vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    2x Streptococcus pyogenes sgRNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CDKN1A sgRNA

    Plasmid

    #138189

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDKN1A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas9-sgRNA-A

    Plasmid

    #149369

    Purpose

    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    Cas9
    sgRNA-A
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    3xFLAG, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CBh, PGK, and U6

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. HaloKu70 sgRNA

    Plasmid

    #207583

    Purpose

    sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    GAGCAGTAGCCAACATGTCA
    

    Expression

    Mammalian

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. LC3B sgRNA

    Plasmid

    #207556

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the LC3B locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CACTGAATACCAGCGCCTAG
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcU6_1 sgRNA

    Plasmid

    #92395

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.1 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    chick U6.1

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCB2-sgRNA

    Plasmid

    #129463

    Purpose

    Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    pgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. ULK1 sgRNA

    Plasmid

    #207559

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    CCAGCCAGGCCAGAAAGGTC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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