|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||112645||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerFillip Port and Simon Bullock
- Backbone size w/o insert (bp) 6700
- Total vector size (bp) 7791
Vector typeInsect Expression
Growth in Bacteria
Gene/Insert nameU6:3-tRNA-sgRNA expression cassette
SpeciesD. melanogaster (fly)
Insert Size (bp)1000
Terms and Licenses
Plasmid by Fillip Port and Michael Boutros.
A detailed cloning protocol is available at http://crisprflydesign.org/grna-expression-vectors/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFD5_w was a gift from Michael Boutros (Addgene plasmid # 112645 ; http://n2t.net/addgene:112645 ; RRID:Addgene_112645)