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In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9.
Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F
Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
60957PX551pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
60958PX552pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

Antibodies from Article