Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 14,183 results for: pET

Showing: 6181 - 6200 of 14183 results

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pET21d-colEdes15/Imdes15

Plasmid

#107123

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des15

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes15/Imdes15

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes16/Imdes16

Plasmid

#107124

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des16

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes16/Imdes16

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes17/Imdes17

Plasmid

#107125

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes17/Imdes17

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes18/Imdes18

Plasmid

#107126

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes18/Imdes18

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes1/Imdes1

Plasmid

#107108

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des1

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes1/Imdes1

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes2/Imdes2

Plasmid

#107109

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes2/Imdes2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes3/Imdes3

Plasmid

#107110

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3/Imdes3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes3.5/Imdes3.5

Plasmid

#107111

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3.5/Imdes3.5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes4/Imdes4

Plasmid

#107112

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes4/Imdes4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes5/Imdes5

Plasmid

#107113

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes5/Imdes5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes6/Imdes6

Plasmid

#107114

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

Depositor

Sarel Fleishman

Publication

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes6/Imdes6

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)_MBP-nLucCP-65/68

Plasmid

#113921

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/66

Plasmid

#113920

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/66

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)-LUCIDRS-MTX

Plasmid

#113925

Purpose

Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

LUCID_RS-MTX

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-clbI S178A

Plasmid

#114159

Purpose

Expresses ClbI S178A

Depositor

Emily Balskus

Publication

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbI

Tags

6xHis and 6xHis-Thrombin site

Expression

Bacterial

Mutation

Mutated S178 to Ala

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Publication

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P46A)

Plasmid

#112721

Purpose

This mutation (P46A) marks the first residue within the evolved region of U1A.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P46A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P46A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6181 - 6200 of 14183 results

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