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pLyGo-Ec-4

Plasmid

#163130

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-3

Plasmid

#163129

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-5

Plasmid

#163131

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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PP318

Plasmid

#78941

Purpose

Expresses GFP. Figure 3. Strain 5.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP297

Plasmid

#78937

Purpose

Expresses GFP. Figure 3. Strain 3.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP298

Plasmid

#78938

Purpose

Expresses GFP. Figure 3. Strain 4.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP299

Plasmid

#78939

Purpose

Expresses GFP. Figure 3. Strain 6.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP300

Plasmid

#78940

Purpose

Expresses GFP. Figure 3. Strain 7.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP296

Plasmid

#78936

Purpose

Expresses GFP. Figure 3. Strain 2.

Depositor

Timothy Lu

Publication

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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lvl0-5 [backbone]

Plasmid

#229155

Purpose

5 prime backbone

Depositor

Ron Weiss

Publication

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-3 [backbone]

Plasmid

#229157

Purpose

3 prime backbone

Depositor

Ron Weiss

Publication

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pACYC_EcDRT3

Plasmid

#255316

Purpose

Expresses EcDRT3 from Escherichia coli GF3-3

Depositor

Alex Gao

Publication

Deng et al Science. 2026 Apr 16:eaed1656. doi: 10.1

Insert

EcDRT3 (Drt3a + Drt3b + ncRNA)

Expression

Bacterial

Available since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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(P2)RBSS7

Plasmid

#251372

Purpose

RBS originally used in pDF-lac [74]

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S7

Expression

Bacterial

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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(P4)RBSS2

Plasmid

#251377

Purpose

RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S2

Expression

Bacterial

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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(P6)RBSS2

Plasmid

#251387

Purpose

RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S2

Expression

Bacterial

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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(P6)RBSS3

Plasmid

#251388

Purpose

RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis)

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S3

Expression

Bacterial

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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(P6)RBSS5

Plasmid

#251390

Purpose

RBS from rbcL gene; slr0090 (Synechocystis)

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S5

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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(P6)RBSS7

Plasmid

#251392

Purpose

RBS originally used in pDF-lac [74]

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S7

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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(P4)RBSS1

Plasmid

#251376

Purpose

Synthetic RBS based on Synechocystis ribosome 30S anti-SD

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S1

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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(P6)RBSS1

Plasmid

#251386

Purpose

Synthetic RBS based on Synechocystis ribosome 30S anti-SD

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S1

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge