CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.
Venero OM, Ndeh R, Pan A, Patrikainen P, Eriksson M, Akhgari A, Kakko L, Nedorezova D, Mohamed O, Thomson L, Bonde A, Kelanne N, Aro EM, Adamala KP, Kallio P
BMC Biotechnol. 2026 Feb 16;26(1):38. doi: 10.1186/s12896-025-01098-7.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251363 | pDFC(P0) | Replicative expression plasmid (shuttle vector) backbone for CyanoConstruct system. |
| 251364 | pSIC(P0) | Backbone for construct integration in Synechocystis locus slr1311 for CyanoConstruct system. |
| 251365 | (P1)PA1lacO1 | IPTG-inducible Plac promoter variant with lacIq repressor and RiboJ |
| 251366 | (P2)RBSS1 | Synthetic RBS based on Synechocystis ribosome 30S anti-SD |
| 251367 | (P2)RBSS2 | RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002 |
| 251368 | (P2)RBSS3 | RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis) |
| 251369 | (P2)RBSS4 | RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis) |
| 251370 | (P2)RBSS5 | RBS from rbcL gene; slr0090 (Synechocystis) |
| 251371 | (P2)RBSS6 | Synthetic RBS used in expression in Synechocystis |
| 251372 | (P2)RBSS7 | RBS originally used in pDF-lac [74] |
| 251373 | (P2)RBSB | RBS designed in silico [75] for high expression in E. coli |
| 251374 | (P3)syfp2 | Gene encoding yellow fluorescent protein variant |
| 251375 | (P3)Adh | Gene encoding aldehyde reductase slr1192 (Synechocystis) |
| 251376 | (P4)RBSS1 | Synthetic RBS based on Synechocystis ribosome 30S anti-SD |
| 251377 | (P4)RBSS2 | RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002 |
| 251378 | (P4)RBSS3 | RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis) |
| 251379 | (P4)RBSS4 | RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis) |
| 251380 | (P4)RBSS5 | RBS from rbcL gene; slr0090 (Synechocystis) |
| 251381 | (P4)RBSS6 | Synthetic RBS used in expression in Synechocystis |
| 251382 | (P4)RBSS7 | RBS originally used in pDF-lac [74] |
| 251383 | (P4)RBSB | RBS designed in silico [75] for high expression in E. coli |
| 251384 | (P5)efe | Gene encoding Ethylene Forming Enzyme (Pseudomonas syringae) |
| 251385 | (P5)pdc | Gene encoding pyruvate decarboxylase (Zymomonas mobilis) |
| 251386 | (P6)RBSS1 | Synthetic RBS based on Synechocystis ribosome 30S anti-SD |
| 251387 | (P6)RBSS2 | RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002 |
| 251388 | (P6)RBSS3 | RBS from C-phycocyanin beta gene cpcB; sll1577 (Synechocystis) |
| 251389 | (P6)RBSS4 | RBS from photosystem II D1 gene psbA2; slr1311 (Synechocystis) |
| 251390 | (P6)RBSS5 | RBS from rbcL gene; slr0090 (Synechocystis) |
| 251391 | (P6)RBSS6 | Synthetic RBS used in expression in Synechocystis |
| 251392 | (P6)RBSS7 | RBS originally used in pDF-lac [74] |
| 251393 | (P6)RBSB | RBS designed in silico [75] for high expression in E. coli |
| 251394 | (P6)TT | rrnB T1 terminator derived from pTrc99A |
| 251395 | (P7)Pk | Gene encoding pyruvate kinase (Enterococcus faecalis) |
| 251396 | (P8)TT | rrnB T1 terminator derived from pTrc99A |
| 253857 | (P4)TT | rrnB T1 terminator derived from pTrc99A |