We narrowed to 13,794 results for: CAR

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GST-TEVcs-GFP-ATG101

Plasmid

#171415

Purpose

For the purifcation of GFP-ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ATG101 (ATG101 Synthetic)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab5 DN

Plasmid

#37694

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S43N (dominant negative)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pACYC-OspCas12c-noncoding

Plasmid

#120878

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-OspCas12

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D_dC

Plasmid

#176466

Purpose

express VPS13D lacking C-terminal 407 aa in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Tags

GFP-Ub

Expression

Mammalian

Mutation

C-terminal 407 aa is deleted.

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
GST-GFP-ATG14

Plasmid

#171426

Purpose

For the purifcation of GFP- PI3KC3 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ATG14 (ATG14 Human)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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M49 GST-TEV-Chibby-C

Plasmid

#17197

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 64-126

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab5 CA

Plasmid

#37702

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q88L (constitutive active)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
M47 MBP-TEV-Chibby

Plasmid

#17195

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

MBP

Expression

Bacterial

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-EGFP-RIP2_S176A

Plasmid

#131202

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

Insert

RIPK2 S176A (RIPK2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S176A

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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psbA2(noATbox)-PHLS (c)

Plasmid

#52308

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Mutation

CAAATACA box in the psbA2 promoter to ggcgcgcc

Promoter

psbA2

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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JPF0522

Plasmid

#124013

Purpose

Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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cpc-PHLS (g)

Plasmid

#52311

Purpose

Replaces the cpc operon in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Synechocystis endogenous cpc-operon promoter

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab11 WT

Plasmid

#37711

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab7 DN

Plasmid

#44826

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

T22N (dominant negative)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170811

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD1_1

Plasmid

#36365

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only