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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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182,244 results

Showing: 6481 - 6500 of 182244 results

  3. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100902-plasmid-map-sequence-id-195323

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  5. pSNAP-tag (T7) Vector

    Plasmid
    #101137
    Purpose
    Expression of protein of interest with SNAP-Tag in bacterial cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    SNAP-tag (SNAP26b)
    Expression
    Bacterial
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    101137-plasmid-map-sequence-id-194162

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  12. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid
    #71293
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Publication
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71293-plasmid-map-sequence-id-127936

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  19. pFRT-TODestGFP_RNAseH1

    Plasmid
    #65784
    Purpose
    expresses GFP-tagged RNAseH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Tags
    GFP and V5
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65784-plasmid-map-sequence-id-111845

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Showing: 6481 - 6500 of 182244 results