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(Plasmid #104585)


Item Catalog # Description Quantity Price (USD)
Plasmid 104585 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    UCP1 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Ucp1 (a.k.a. Slc25a7, Ucp)
  • Promoter UCP1
  • Tag / Fusion Protein
    • TurboGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CMV promoter driving GFP expression was removed and replaced with the -5.5 kb mouse UCP1 promoter + Kozak

Reference sequence for promoter may be found here:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -5.5kb-UCP1-GFP was a gift from Irfan Lodhi (Addgene plasmid # 104585 ; ; RRID:Addgene_104585)
  • For your References section:

    PexRAP Inhibits PRDM16-Mediated Thermogenic Gene Expression. Lodhi IJ, Dean JM, He A, Park H, Tan M, Feng C, Song H, Hsu FF, Semenkovich CF. Cell Rep. 2017 Sep 19;20(12):2766-2774. doi: 10.1016/j.celrep.2017.08.077. 10.1016/j.celrep.2017.08.077 PubMed 28930673