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(Plasmid #70185)


Item Catalog # Description Quantity Price (USD)
Plasmid 70185 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 7150
  • Modifications to backbone
    Replaced Puromycin resistance gene with GpNLuc LumiFluor.
  • Vector type
    Mammalian Expression, Retroviral
  • Promoter PGK

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTGGGCTCAGAGGCTGGG
  • 3′ sequencing primer CCTACAGGTGGGGTCTTTC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroX-Tight-MCS_PGK-GpNLuc was a gift from Antonio Amelio (Addgene plasmid # 70185 ; ; RRID:Addgene_70185)
  • For your References section:

    Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis. Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, Cleveland JL, Amelio AL. Cancer Res. 2015 Sep 30. pii: canres.3538.2014. 10.1158/0008-5472.CAN-14-3538 PubMed 26424696