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pT7-CalfluxVTN
(Plasmid #83926)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83926 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSETb
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2787
  • Total vector size (bp) 4224
  • Modifications to backbone
    The T7-tag, Xpress tag and Multiple Cloning Site of pRSETb was removed via restriction digestion of the NheI and HindIII sites. An EcoRI site was inserted downstream of the new NheI site, and CalfluxVTN was inserted via the new EcoRI and HindIII restriction sites.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CalfluxVTN
  • Alt name
    Venus-TnC-NanoLuc
  • Species
    Synthetic
  • Insert Size (bp)
    1437
  • Mutation
    None
  • GenBank ID
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-CalfluxVTN was a gift from Carl Johnson (Addgene plasmid # 83926)
  • For your References section:

    Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Yang J, Cumberbatch D, Centanni S, Shi SQ, Winder D, Webb D, Johnson CH. Nat Commun. 2016 Oct 27;7:13268. doi: 10.1038/ncomms13268. 10.1038/ncomms13268 PubMed 27786307