|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83926||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2787
- Total vector size (bp) 4224
Modifications to backboneThe T7-tag, Xpress tag and Multiple Cloning Site of pRSETb was removed via restriction digestion of the NheI and HindIII sites. An EcoRI site was inserted downstream of the new NheI site, and CalfluxVTN was inserted via the new EcoRI and HindIII restriction sites.
Vector typeBacterial Expression
Growth in Bacteria
Insert Size (bp)1437
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT7-CalfluxVTN was a gift from Carl Johnson (Addgene plasmid # 83926)
For your References section:Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Yang J, Cumberbatch D, Centanni S, Shi SQ, Winder D, Webb D, Johnson CH. Nat Commun. 2016 Oct 27;7:13268. doi: 10.1038/ncomms13268. 10.1038/ncomms13268 PubMed 27786307