PurposeExpress a lysosomal Tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102931||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
SpeciesR. norvegicus (rat)
- Promoter ubc
/ Fusion Protein
- mRFP-2xFlag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcorI (not destroyed)
- 5′ sequencing primer CGAAGGAATAGAAGAAGAAGGTGGAGA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
LAMP1-RFP was cloned from Plasmid #34611
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLJC5-LAMP1-RFP-2xFlag was a gift from David Sabatini (Addgene plasmid # 102931 ; http://n2t.net/addgene:102931 ; RRID:Addgene_102931)
For your References section:Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes. Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, Freinkman E, Sabatini DM. Science. 2017 Nov 10;358(6364):807-813. doi: 10.1126/science.aan6298. Epub 2017 Oct 26. 10.1126/science.aan6298 PubMed 29074583