168,321 results

1. V5-miniTurbo-NES_pCDNA3

   Plasmid

   #107170

   Purpose

   expresses V5-tagged miniTurbo in the mammalian cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR P2R-P3-EGFP

   Plasmid

   #48349

   Purpose

   Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Mutation

   Aminoacid 40 is valine in our sequence, while it …

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. GGE0029

   Plasmid

   #120730

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Type

   Empty backbone

   Use

   Cloning/assembly plasmid

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-iCAP-AAV.MaCPNS2

   Plasmid

   #185137

   Purpose

   non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

   Insert

   Synthetic construct isolate AAV-MaCPNS2 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAIDFA-EF1a-NmScarlet-mAID

   Plasmid

   #140615

   Purpose

   Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1 mutated F74A
   

   Expression

   Mammalian

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCURE

   Plasmid

   #23013

   Depositor

   James Bowie

   Article

   Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

   Insert

   I-CreI homing endonuclease
   

   Expression

   Bacterial

   Mutation

   I-CreI expression is controlled by the arabinose …

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pMEL13

   Plasmid

   #107919

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDESTmycQKI

   Plasmid

   #19870

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   quaking homolog, KH domain RNA binding (QKI Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pH2B-miRFP680-N1

    Plasmid

    #197229

    Purpose

    Expresses the fusion protein of H2B-miRFP680 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    H2B-miRFP680
    

    Expression

    Mammalian

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CVS-N2c-nl.EGFP-jRGECO1a

    Plasmid

    #172386

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + jRGECO1a
    

    Use

    Neurotropic virus

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. tdTomato-Lysosomes-20

    Plasmid

    #58109

    Purpose

    Localization: Lysosome Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pSLQ-mgU2-47

    Plasmid

    #127585

    Purpose

    Expresses mgU2-47 scaRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    mgU2-47 scaRNA
    

    Expression

    Mammalian

    Promoter

    modified U6

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM37443

    Plasmid

    #153218

    Purpose

    Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS426 TEF mCherry

    Plasmid

    #129433

    Purpose

    express mcherry

    Depositor

    Oskar Laur

    Insert

    mcherry
    

    Expression

    Yeast

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLIX403 RNaseH1

    Plasmid

    #237695

    Purpose

    Inducible lentiviral expression of RNaseH1

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    RNaseH1 (RNASEH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-syn-FLEX-jYCaMP1s

    Plasmid

    #135420

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-HA-PD-L1

    Plasmid

    #121492

    Purpose

    Expression of human PD-L1 WT in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMEL12

    Plasmid

    #107918

    Purpose

    hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. GW1-Peredox-mCherry

    Plasmid

    #32380

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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