161,729 results

1. pDONR223-LOC340371

   Plasmid

   #23545

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   LOC340371 (NRBP2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pF2-DTA

   Plasmid

   #42521

   Depositor

   Terry Magnuson

   Article

   Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

   Insert

   EF1a-DTA
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-HRP-C

   Plasmid

   #40163

   Depositor

   Paul Ortiz de Montellano

   Article

   Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

   Insert

   Horseradish peroxidase
   

   Use

   Coli cloning vector

   Tags

   Six histidine

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZFX-donor

   Plasmid

   #112366

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZFX with GFP

   Depositor

   Kevin White

   Insert

   ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

   Plasmid

   #141386

   Purpose

   Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-M (M SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pFH2.2_mCherry-MS2x12

   Plasmid

   #205521

   Purpose

   Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. [#GS5-RV] MitoTRACER-Donor

   Plasmid

   #233505

   Purpose

   Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025; In Press.

   Insert

   MitoTRACER-Donor
   

   Use

   Retroviral and Synthetic Biology

   Tags

   GFP11 and HA Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. [#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

   Plasmid

   #233506

   Purpose

   Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025; In Press.

   Insert

   DsRed loxp eGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. [#GS7-LV] DsRed GFP TEVp Zeo Switch - Recipient

   Plasmid

   #233507

   Purpose

   Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025; In Press.

   Insert

   DsRed loxP eGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5_mEGFP-TAZ-CAMTA1-KS

    Plasmid

    #237677

    Purpose

    For overexpression of mEGFP-TAZ-CAMTA1-KS

    Depositor

    Denes Hnisz

    Insert

    TAZ-CAMTA1-KS (WWTR1, CAMTA1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. C1-MPAct-mRuby3

    Plasmid

    #155221

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRL277

    Plasmid

    #70255

    Purpose

    sacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

    Depositor

    Peter Wolk

    Article

    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

    Type

    Empty backbone

    Use

    Conditionally lethal

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-hARdelta538-614

    Plasmid

    #89107

    Purpose

    mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    hAR-538-614 (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR delta 538-614

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pACYC-Taq

    Plasmid

    #102793

    Purpose

    Expresses Taq DNA polymerase under WT T7 promoter

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    Taq DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-mScarlet-Zeo

    Plasmid

    #207733

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSETa mEos2

    Plasmid

    #20341

    Purpose

    bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

    Depositor

    Loren Looger

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    mEos-2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTMB304_ZFcharm_Kv1_Prnp_SCR

    Plasmid

    #220842

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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