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peSpCas9(1.1)-2×sgRNA (empty, donor)

Plasmid

#80768

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-N-FLAG-HA-TBK1

Plasmid

#131791

Purpose

Lentiviral vector expressing WT TBK1

Depositor

Alicia Pickrell

Article

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

N-FLAG-HA

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRC0940 (circRNA entry vector)

Plasmid

#189967

Purpose

Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMD1 anti-GFP nanobody-Tn5

Plasmid

#215567

Purpose

Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

Depositor

Isabelle Mansuy

Article

Maria A. et al bioRxiv 2024.07.12.603224

Insert

anti-GFP nanobody-Tn5 fusion

Tags

3xFlag and intein-CBD

Expression

Bacterial

Promoter

T7

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Myristoylated optoDroplet

Plasmid

#111507

Purpose

For membrane localized optoDroplets

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

Cry2 (CRY2 Mustard Weed)

Use

Lentiviral

Tags

FUS, Fusion Red, and Myristoylation Sequence (MYR…

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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HA-Clover-HIF-1alpha Wildtype

Plasmid

#163365

Purpose

Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

Depositor

Violaine Sée

Insert

Hypoxia Inducible Factor 1 alpha (HIF1A Human)

Tags

HA- and Clover-tagged

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV Promoter

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CRX-T2A-NeuroD1

Plasmid

#194607

Purpose

Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

Depositor

Haruhisa Inoue

Article

Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

Insert

CRX-T2A-NeuroD1

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCBC-DT2T3

Plasmid

#50591

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T2, gRNA scaffold, U6-29t plus, U6-1p, T3

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-CD63-L7Ae

Plasmid

#180503

Purpose

Human CD63-L7Ae fusion protein

Depositor

Masaharu Somiya

Article

Somiya et al bioRxiv 2022.01.25.477620

Insert

Human CD63-L7Ae fusion protein

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

Pooled Library

#136479

Purpose

Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

Depositor

Jonathan Weissman

Article

Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Mutation

Y40N

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML1-G-GECO1.2-ERES

Plasmid

#207144

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Mcoln1 (MCOLN1 Human)

Tags

ER-Export Signal (ERES) and G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-Golgi

Plasmid

#36205

Purpose

In vivo visualization of golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Golgi targeting sequence (B4GALT1 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI-2Psub-Kv-WPRE

Plasmid

#247223

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIa

Depositor

Francois St-Pierre

Article

Carolan et al Nat Commun. 2025 Oct 3;16(1):8834. doi:

Insert

JEDI-2Psub-Kv

Use

AAV

Tags

Kv

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-U6-SIPZ

Plasmid

#174848

Purpose

empty vector for cloning shRNA and/or miRNA

Depositor

Helen Morrison

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-A3-HA-ubiquitin

Plasmid

#220145

Purpose

Expresses A3-Ub for E3 substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

Ubiquitin C (UBC Human)

Tags

A3-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pC0056 - LwCas13a-msfGFP-NES

Plasmid

#105815

Purpose

Expresses active LwaCas13a-NES for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR and Lentiviral

Tags

NES and msfGFP-NES-3xHA

Expression

Mammalian

Promoter

EF-1a

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

Plasmid

#136262

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mNeonGreen-NLS

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

Plasmid Kit

#1000000171

Purpose

MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants.

Depositor

Sylvestre Marillonnet, Johannes Stuttmann

Application

Cloning and Synthetic Biology, Genome Editing

Vector Type

Plant Expression

Editing Type

CRISPR

Cloning Type

Golden Gate (MoClo)

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only
7TGP

Plasmid

#24305

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-eGFP // SV40-PuroR

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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