Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

1481 pcDNA3 flag HA 14-3-3 zeta
(Plasmid #9002)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 9002 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 zeta
  • Alt name
    14-3-3
  • Alt name
    YWHAZ
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    750
  • Mutation
    T226A
  • GenBank ID
    NM_174814
  • Entrez Gene
    YWHAZ
  • Tags / Fusion Proteins
    • flag (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Created by Francisca Vazquez.

A T226A point mutation is found when Addgene's QC results are aligned with the noted Genbank sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1481 pcDNA3 flag HA 14-3-3 zeta was a gift from William Sellers (Addgene plasmid # 9002 ; http://n2t.net/addgene:9002 ; RRID:Addgene_9002)