pcDNA3 HA 14-3-3 sigma
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11946||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt name14-3-3 sigma
SpeciesH. sapiens (human)
Entrez GeneSFN (a.k.a. YWHAS)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3 HA 14-3-3 sigma was a gift from Michael Yaffe (Addgene plasmid # 11946 ; http://n2t.net/addgene:11946 ; RRID:Addgene_11946)
For your References section:A structural basis for 14-3-3sigma functional specificity. Wilker EW, Grant RA, Artim SC, Yaffe MB. J Biol Chem. 2005 May 13. 280(19):18891-8. 10.1074/jbc.M500982200 PubMed 15731107