PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||188779||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepMH0001, Addgene # 85969
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Promoter SFFV
/ Fusion Protein
- Zim3 KRAB-NLS fusion (N terminal on insert)
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Human lentiviral vector for expression of dCas9 with an N-terminal Zim3 KRAB-NLS fusion, a C-terminal HA-2xNLS, and EGFP linked by a P2A site from an EF1a promoter with an upstream ubiquitous chromatin opening element. pNM1123.
Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry was a gift from Marco Jost & Jonathan Weissman (Addgene plasmid # 188779 ; http://n2t.net/addgene:188779 ; RRID:Addgene_188779)
For your References section:Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors. Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M. Elife. 2022 Dec 28;11:e81856. doi: 10.7554/eLife.81856. 10.7554/eLife.81856 PubMed 36576240