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A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells.
Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, Eggan K, Barrett LE
Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
PubMed Article

Plasmids from Article

ID Plasmid  
128467S1_AscI_V5-gateway_Bsu36I
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128468S2_AscI_HA-gateway_Bsu36I
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128469S5_EcoRV_Ef1a_AscI
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128470S6_BstBI_SV40_SwaI
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128471S7_SwaI_Zeocin_PacI
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128472S8_attL1-N-term-EGFP-attL2
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128473S9_attL1-EGFP-C-term-attL2
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128474S10_EcoRV_hcSYN_AscI
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128475S11_EcoRV_CAMKIIA_AscI
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128476S12_EcoRV_PGK_AscI
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128477S13_EcoRV_UBC_AscI
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137865pPN454
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137866pPN434
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137867pPN435
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137868pPN446
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137869pPN431
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137870pPN432
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137871pPN447
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137872pPN455
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137873pPN441
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137874pPN440
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137875pPN459
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137876pPN458
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137877pPN461
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137878pPN460
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137879pT077
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137880pT076
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137882pGEP179_pX330K
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