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A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells.
Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, Eggan K, Barrett LE
Sci Rep. 2020 Jan 20;10(1):635. doi: 10.1038/s41598-020-57500-1.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
128467S1_AscI_V5-gateway_Bsu36I destination vector with N-terminal V5 tag
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128468S2_AscI_HA-gateway_Bsu36I destination vector with N-terminal HA tag
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128469S5_EcoRV_Ef1a_AscI Ef1a promoter
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128470S6_BstBI_SV40_SwaISV40 promoter
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128471S7_SwaI_Zeocin_PacI Zeocin Resistance gene
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128472S8_attL1-N-term-EGFP-attL2EGFP entry clones
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128473S9_attL1-EGFP-C-term-attL2 EGFP entry clone
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128474S10_EcoRV_hcSYN_AscI hcSYN promoter
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128475S11_EcoRV_CAMKIIA_AscI CAMKII1 promoter
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128476S12_EcoRV_PGK_AscI PGK promoter
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128477S13_EcoRV_UBC_AscI UBC promoter
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137865pPN454Expression of gRNA i1 targeting TCF4 for CRISPRi; U6 promoter
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137866pPN434Expression of gRNA i2 targeting TCF4 for CRISPRi; U6 promoter
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137867pPN435Expression of gRNA i3 targeting TCF4 for CRISPRi; U6 promoter
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137868pPN446Expression of gRNA a1 targeting TCF4 for CRISPRa; U6 promoter
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137869pPN431Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter
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137870pPN432Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter
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137871pPN447Expression of gRNA targeting POGZ for CRISPRi; U6 promoter
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137872pPN455Expression of gRNA targeting POGZ for CRISPRa; U6 promoter
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137873pPN441PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR
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137874pPN440PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR
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137875pPN459PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR
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137876pPN458PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR
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137877pPN461PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR
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137878pPN460PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR
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137879pT077Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP
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137880pT076Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP
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137881pGEP163PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR
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137882pGEP179_pX330KEntry vector for multi-gRNA cloning
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