332,339 results

1. PB-FLuc+GFPd2

   Plasmid

   #127190

   Purpose

   Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)

   Depositor

   Jordan Green

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-FLuc+GFPd2

   Plasmid

   #127190

   Purpose

   Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)

   Depositor

   Jordan Green

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

   Plasmid

   #188384

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

   Plasmid

   #188384

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-cFos-tTA-pA

   Plasmid

   #66794

   Purpose

   Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   cfos promoter linked to the tet activator gene
   

   Use

   AAV

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-cFos-tTA-pA

   Plasmid

   #66794

   Purpose

   Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   cfos promoter linked to the tet activator gene
   

   Use

   AAV

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #126217

   Purpose

   Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV9)

    Viral Prep

    #100848-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Tags

    None

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV9)

    Viral Prep

    #100848-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Tags

    None

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid

    #73333

    Purpose

    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid

    #73333

    Purpose

    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTK645

    Plasmid

    #114711

    Purpose

    Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA(1700-2115)-RFP-Nano
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK645

    Plasmid

    #114711

    Purpose

    Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    NuMA(1700-2115)-RFP-Nano
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge