168,493 results

1. pcDNA3.1(+)IRES GFP

   Plasmid

   #51406

   Purpose

   Expresses GFP from internal ribosome entry sequence, cloning component

   Depositor

   Kathleen_L Collins

   Article

   Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

   Insert

   IRES GFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Expression

   Mammalian

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF Synaptophysin-mRuby

   Plasmid

   #188980

   Purpose

   Synaptic buton marker

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Synthetic, Rat)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC25A39

   Plasmid

   #131970

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A39 (SLC25A39 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 18, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pHAT2

   Plasmid

   #112583

   Purpose

   Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEG BacMam C term 3C eGFP StrepII

   Plasmid

   #160686

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Tags

   3C eGFP StrepII

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) (AAV PHP.eB)

   Viral Prep

   #163505-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) (#163505). In addition to the viral particles, you will also receive purified AiP11390-pAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1390) plasmid DNA. AAV vector for strong and rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core sequence has 100% sequence conservation between mouse and human. Developed especially for rapid viral labeling in human and NHP organotypic brain slice culture. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Promoter

   minBetaGlobin

   Tags

   SYFP2

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

8. LXRalpha_pcDNA3.1

   Plasmid

   #177621

   Purpose

   Full-length human LXR alpha cDNA sequence in pcDNA3.1+

   Depositor

   Thomas Burris

   Article

   Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

   Insert

   LXR alpha (NR1H3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-FOXJ1

    Plasmid

    #153129

    Purpose

    Expression of Flag tagged human FOXJ1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXJ1 (FOXJ1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-SypHer2-DAAO-NES

    Plasmid

    #119184

    Purpose

    Fusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.

    Depositor

    Thomas Michel

    Article

    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

    Insert

    SypHer2-DAAO-NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRN3P_Kdm5b_MUT

    Plasmid

    #119767

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    Kdm5b MUT
    

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

    Plasmid

    #108912

    Purpose

    Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Has Service

    AAV9

    Insert

    ChroME-ST
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-mGold2s-Calnexin-N-14

    Plasmid

    #231768

    Purpose

    Visualization of ER in mammalian cells using mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s-Calnexin-N-17 (CANX Human)
    

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a.Tat(48-57)-EGFP

    Plasmid

    #165040

    Purpose

    Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    EGFP
    

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

    Plasmid

    #138461

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-VP64-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJAK184

    Plasmid

    #167281

    Purpose

    MazF-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Promoter

    Pxyl

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAC_C_EPHB2

    Plasmid

    #187797

    Purpose

    MAC-tagged gene expression of human EPHB2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHB2 (EPHB2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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