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pKC-4.06
(Plasmid #112084)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112084 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p3xFLAG-CMV-10
  • Backbone size w/o insert (bp) 6204
  • Total vector size (bp) 8717
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3XFLAG firefly luciferase-beta-globin 39PTC
  • Alt name
    FLuc NMD sensor
  • Species
    H. sapiens (human), M. musculus (mouse); Photinus pyralis
  • Insert Size (bp)
    2513
  • Entrez Gene
    HBB (a.k.a. CD113t-C, ECYT6, beta-globin)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    b-globin sequence used in this clone was obtained from Professor Lynne Maquat
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKC-4.06 was a gift from James Inglese (Addgene plasmid # 112084 ; http://n2t.net/addgene:112084 ; RRID:Addgene_112084)
  • For your References section:

    ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg JR. Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178. 33178 [pii] PubMed 29528287